Isolation of β-Amylase Producing Microorganisms

Isolation and Identification of Antibiotic Producing Microorganisms

Microplate Diffusion Assay for Screening of β-Glucanase-Producing Microorganisms

A method is described to screen fungal strains rapidly for overexpression of extracellular β-1,4-endoglucanase in the presence of high levels of sugar compounds. The semi-quantitative assay utilizes microplates in a 96-well format and an azurine dye covalently cross-linked (AZCL) chromogenic substrate. The digestion of AZCL-hydroxyethyl-β-1,4-endoglucanase results in the release of a blue dye d...

Isolation, Identification and Characterization of Lipase Producing Microorganisms from Environment

Objective: Lipases represent an important group of hydrolytic proteins. These enzymes find numerous applications in the industrial sector to provide regioselective molecules of commercial interest. The present investigation was carried out to isolate and identify a bacterium that can quickly produce this enzyme from very simple and cost effective nutrient medium. Materials and Methods: Lipid as...

Screening concepts for the isolation of biosurfactant producing microorganisms.

This chapter gives an overview of current methods for the isolation of biosurfactant producing microbes. The common screening methods for biosurfactants are presented. Sampling and isolation of bacteria are the basis for screening of biosurfactant producing microbes. Hydrocarbon-contaminated sites are the most promising for the isolation of biosurfactant producing microbes, but many strains hav...

Isolation and Optimization of Poly Β Hydroxybutyrate Producing Cyanobacterial Strains

Cyanobacteria have many unexploited potential for natural products with a huge variability in structure and biological activity. Under stress conditions they are reported to produce biopolymers like poly-β-hydroxybutyrate (PHB), which can be produced intracellularly.Cyanobacteria are capable of synthesizing small amount of poly-β-hydroxybutyrate (PHB) under nitrogen and phosphorous starvation c...